Search for a protein in specific genome database

1) Go to NCBI Blast page and select tblastn

2) Select the protein you want to check, for example, I want to check the following protein is present in the database. Select the protein accession and copy it in the query box.

3) Now search for the protein sequence in Database: Whole-genome shotgun contigs (I selected this because Asian Seabass does not have RefSeq Genome) and organism by typing: Asian Seabass

4)  Blast! If you find the protein sequences in the genome, it will appears in the results. 

Note: It will take time, if you are using proteins to search because BLAST has to translate all the 6 frames and search against the database. For faster check of the presence of the sequences in the database, it is better to do a local NCBI blast.