$fastqc filename.fastq
Started analysis of filename.fastq
Exception in thread "Thread-1" java.lang.OutOfMemoryError: Java heap space
at uk.ac.babraham.FastQC.Modules.GCModel.GCModel.<init>(GCModel.java:69)
at uk.ac.babraham.FastQC.Modules.PerSequenceGCContent.processSequence(PerSequenceGCContent.java:198)
at uk.ac.babraham.FastQC.Analysis.AnalysisRunner.run(AnalysisRunner.java:88)
at java.base/java.lang.Thread.run(Thread.java:844)
$ grep -n 'Xm' /home/prakki/sw/FastQC/fastqc
167: unshift @java_args,"-Xmx${memory}m";
170: unshift @java_args,'-Xmx250m';
$ vi /home/prakki/sw/FastQC/fastqc
Changed the 250m to 10g (depends on the RAM of your computer) in the line 170
$ fastqc filename.fastq
Started analysis of filename.fastq
Approx 5% complete for filename.fastq
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Analysis complete for filename.fastq
The most useful tip I've found around. Thanks a lot.
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