Question 1:
I have adata object which does not have layers with spliced and unspliced counts information. Is it even possible one of the kernels from cellrank2 without this information to find the cell fate transition? If yes for above, could you kindly advice what could be used alternative to the spl/unspl count layers?
Yes, we can use pseudotime instead of RNAvelocity to calculate cellfates and transitions.
In cases, where I am not sure of the root celltype - is it possible to use cellrank2?
Yes, using the following command we can:
adata.obsm['X_diffmap'][:, 3].argmax()
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